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Product Info Summary
| SKU: | A15208-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-C7orf47/PPP1R35 Antibody Picoband®
SKU/Catalog Number
A15208-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-C7orf47/PPP1R35 Antibody Picoband® catalog # A15208-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-C7orf47/PPP1R35 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15208-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human C7orf47/PPP1R35 recombinant protein (Position: E27-A253).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15208-1 is reactive to PPP1R35 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
19908 MW
Background of Ppp1r35
PPP1R35 is a putative regulator of protein phosphatase-1. PPP1R35 is a centrosomal protein that regulates centriole elongation and centriole-to-centrosome conversion. Predicted to enable phosphatase binding activity and protein phosphatase inhibitor activity. Predicted to be involved in negative regulation of phosphatase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15208-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human SH-SY5Y whole cell, human K562 whole cell
ICC/IF: U2OS cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of C7orf47/PPP1R35 using anti-C7orf47/PPP1R35 antibody (A15208-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C7orf47/PPP1R35 antigen affinity purified polyclonal antibody (Catalog # A15208-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C7orf47/PPP1R35 at approximately 30 kDa. The expected band size for C7orf47/PPP1R35 is at 28 kDa.
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Figure 2. IF analysis of C7orf47/PPP1R35 using anti-C7orf47/PPP1R35 antibody (A15208-1) and anti-Beta Tubulin antibody (M01857-3).
C7orf47/PPP1R35 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C7orf47/PPP1R35 Antibody (A15208-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of K562 cells using anti-C7orf47/PPP1R35 antibody (A15208-1).
Overlay histogram showing K562 cells stained with A15208-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C7orf47/PPP1R35 Antibody (A15208-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R35 (Source: Uniprot.org, NCBI)
Gene Name
PPP1R35
Full Name
Protein phosphatase 1 regulatory subunit 35
Weight
19908 MW
Superfamily
PPP1R35 family
Alternative Names
Transmembrane protein 240;TMEM240;C1orf70;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R35, check out the PPP1R35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C7orf47/PPP1R35 Antibody Picoband® (A15208-1)
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