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Product Info Summary
| SKU: | A04233 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-C1QB Antibody
SKU/Catalog Number
A04233
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-C1QB Antibody (Catalog # A04233). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C1QB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04233)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human C1QB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04233 is reactive to C1QB in Human
Reconstitution
Calculated molecular weight
26722 MW
Background of C1QB
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Reid K.B.M.; Biochem. J. 231:729-735(1985).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04233 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using C1QB antibody A04233.
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Immunofluorescence analysis of A549 cells, using C1QB antibody A04233.
Protein Target Info & Infographic
Gene/Protein Information For C1QB (Source: Uniprot.org, NCBI)
Gene Name
C1QB
Full Name
Complement C1q subcomponent subunit B
Weight
26722 MW
Alternative Names
Complement C1q subcomponent subunit B;C1QB; C1QB complement C1q B chain complement C1q subcomponent subunit B|complement C1q chain B|complement component 1, q subcomponent, B chain|complement component 1, q subcomponent, beta polypeptide|complement component C1q, B chain|complement subcomponent C1q chain B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on C1QB, check out the C1QB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for C1QB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C1QB Antibody (A04233)
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No publications found for A04233
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-C1QB Antibody
Question
See attached the WB image, lot number and protocol we used for liver using anti-C1QB antibody A04233. Please let me know if you require anything else.
A. Taylor
Verified customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-C1QB antibody A04233. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Is this A04233 anti-C1QB antibody reactive to the isotypes of C1QB?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The immunogen of A04233 anti-C1QB antibody is Synthesized peptide derived from internal of human C1QB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-07
Question
My lab would like to test anti-C1QB antibody A04233 on human liver for research purposes, then I may be interested in using anti-C1QB antibody A04233 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The products we sell, including anti-C1QB antibody A04233, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-C1QB antibody A04233 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-C1QB antibody (A04233) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
Will anti-C1QB antibody A04233 work on horse IF with brain?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Our lab technicians have not tested anti-C1QB antibody A04233 on horse. You can run a BLAST between horse and the immunogen sequence of anti-C1QB antibody A04233 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-29
Question
Is a blocking peptide available for product anti-C1QB antibody (A04233)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
We do provide the blocking peptide for product anti-C1QB antibody (A04233). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-24
Question
Would A04233 anti-C1QB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
As indicated on the product datasheet, A04233 anti-C1QB antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-10
Question
My question regarding product A04233, anti-C1QB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Li
Verified customer
Asked: 2018-03-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04233 anti-C1QB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-19
Question
Do you have a BSA free version of anti-C1QB antibody A04233 available?
P. Kulkarni
Verified customer
Asked: 2016-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C1QB antibody A04233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-01
Question
I was wanting to use your anti-C1QB antibody for IF for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
R. Brown
Verified customer
Asked: 2015-07-06
Answer
As indicated on the product datasheet, A04233 anti-C1QB antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-06
Question
Does anti-C1QB antibody A04233 work for IF with liver?
P. Jackson
Verified customer
Asked: 2014-09-09
Answer
According to the expression profile of liver, C1QB is highly expressed in liver. So, it is likely that anti-C1QB antibody A04233 will work for IF with liver.
Boster Scientific Support
Answered: 2014-09-09
Question
I see that the anti-C1QB antibody A04233 works with IF, what is the protocol used to produce the result images on the product page?
C. Mitchell
Verified customer
Asked: 2013-04-19
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-19
