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Product Info Summary
| SKU: | M00297-5 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374
SKU/Catalog Number
M00297-5
Size
100uL
Form
Liquid
Description
Boster Bio Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (Catalog # M00297-5). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM374
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to N-terminus of human Proto-oncogene c-Fos
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Proto-oncogene c-Fos. It may also react to mouse and rat c-Fos, as predicted by immunogen homology.
Reactive Species
M00297-5 is reactive to FOS in Human
Reconstitution
Calculated molecular weight
40695 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00297-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:5000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cell lysates: non-treated (-) or treated (+) with TPA, using anti-TTF1 rabbit monoclonal antibody (Clone RM374) at a 1:5000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-c-Fos rabbit monoclonal antibody (Clone RM374) at a 1:1250 dilution.
Protein Target Info & Infographic
Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)
Gene Name
FOS
Full Name
Proto-oncogene c-Fos
Weight
40695 MW
Superfamily
bZIP family
Alternative Names
Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7; FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOS, check out the FOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (M00297-5)
Hello CJ!
M00297-5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374
Question
Is a blocking peptide available for product anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5)?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We do provide the blocking peptide for product anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-31
Question
Do you have a BSA free version of anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 available?
T. Moore
Verified customer
Asked: 2020-02-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-25
Question
We are interested in using your anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 for response to muscle stretch studies. Has this antibody been tested with western blotting on thyroid cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you for your inquiry. This M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 is validated on thyroid cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5. Let me know if you need anything else.
P. Li
Verified customer
Asked: 2019-07-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-03
Question
Our team were satisfied with the WB result of your anti-c-Fos Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Based on literature, pancreas does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-05
Question
I see that the anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-21
Question
Does M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
As indicated on the product datasheet, M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-16
Question
you antibody to test anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 on human pancreas for research purposes, then I may be interested in using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
The products we sell, including anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-09
Question
We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 supposed to stain leukemic t-cell positively?
K. Bhatt
Verified customer
Asked: 2019-01-21
Answer
According to literature leukemic t-cell does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-21
Question
Here is the WB image, lot number and protocol we used for pancreas using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-27
Question
Will anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 work for WB with pancreas?
D. Miller
Verified customer
Asked: 2018-02-26
Answer
According to the expression profile of pancreas, FOS is highly expressed in pancreas. So, it is likely that anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 will work for WB with pancreas.
Boster Scientific Support
Answered: 2018-02-26
Question
Is this M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 reactive to the isotypes of FOS?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
The immunogen of M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 is A peptide corresponding to N-terminus of human Proto-oncogene c-Fos. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-07
Question
We are currently using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
C. Taylor
Verified customer
Asked: 2016-08-17
Answer
The anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-17
Question
I was wanting to use your anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
J. Baker
Verified customer
Asked: 2015-04-14
Answer
It shows on the product datasheet, M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-14
Question
I have a question about product M00297-5, anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Evans
Verified customer
Asked: 2014-10-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-03
Question
We have tried in the past anti-c-Fos Rabbit Monoclonal antibody, Clone
Z. Johnson
Verified customer
Asked: 2014-02-07
Answer
I have checked the website and datasheets of our anti-c-Fos Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2014-02-07
