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Product Info Summary
| SKU: | M01564 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E3]
SKU/Catalog Number
M01564
Size
100 µl
Description
Boster Bio BuB1B (BubR1) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3). Catalog# M01564. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01564)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI6E3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01564 is reactive to BUB1B in Dog, Human, Monkey, Rat
Reconstitution
Calculated molecular weight
119.545kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01564 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either BUB1B (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BUB1B antibody (M01564)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-BUB1B mouse monoclonal antibody (M01564) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For BUB1B (Source: Uniprot.org, NCBI)
Gene Name
BUB1B
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Weight
119.545kDa
Superfamily
protein kinase superfamily
Alternative Names
Bub1A; BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta; budding uninhibited by benzimidazoles 1 homolog beta (yeast); EC 2.7.11.1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3LBUB1beta; Mitotic checkpoint kinase MAD3L; Protein SSK1; SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1B BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1 BUB1 mitotic checkpoint serine/threonine kinase B mitotic checkpoint serine/threonine-protein kinase BUB1 beta|BUB1B, mitotic checkpoint serine/threonine kinase|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1 homolog beta|mitotic checkpoint kinase MAD3L
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BUB1B, check out the BUB1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BUB1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: OTI6E3] (M01564)
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