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Product Info Summary
| SKU: | A01564 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BubR1 BUB1B Antibody
SKU/Catalog Number
A01564
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BubR1 BUB1B Antibody catalog # A01564. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BubR1 BUB1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01564)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human BUB1B. AA range:341-390
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01564 is reactive to BUB1B in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
119545 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01564 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using BubR1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For BUB1B (Source: Uniprot.org, NCBI)
Gene Name
BUB1B
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Weight
119545 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta;2.7.11.1;MAD3/BUB1-related protein kinase;hBUBR1;Mitotic checkpoint kinase MAD3L;Protein SSK1;BUB1B;BUBR1, MAD3L, SSK1; BUB1B BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1 BUB1 mitotic checkpoint serine/threonine kinase B mitotic checkpoint serine/threonine-protein kinase BUB1 beta|BUB1B, mitotic checkpoint serine/threonine kinase|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1 homolog beta|mitotic checkpoint kinase MAD3L
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BUB1B, check out the BUB1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BUB1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BubR1 BUB1B Antibody (A01564)
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No publications found for A01564
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Customer Q&As
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5 Customer Q&As for Anti-BubR1 BUB1B Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-BubR1 antibody A01564. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this A01564 anti-BubR1 antibody reactive to the isotypes of BUB1B?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The immunogen of A01564 anti-BubR1 antibody is Synthesized peptide derived from the Internal region of human BubR1. at AA rangle: 310-390. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-01
Question
Can you help my question with product A01564, anti-BubR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01564 anti-BubR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-10
Question
Does anti-BubR1 antibody A01564 work for WB with cervix carcinoma?
C. Johnson
Verified customer
Asked: 2016-05-05
Answer
According to the expression profile of cervix carcinoma, BUB1B is highly expressed in cervix carcinoma. So, it is likely that anti-BubR1 antibody A01564 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2016-05-05
Question
I was wanting to use your anti-BubR1 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
G. Banerjee
Verified customer
Asked: 2016-03-02
Answer
You can see on the product datasheet, A01564 anti-BubR1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-02
