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Product Info Summary
| SKU: | A04100 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BTF Antibody
SKU/Catalog Number
A04100
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BTF Antibody catalog # A04100. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BTF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04100)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human BTF. at AA rangle: 250-330
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04100 is reactive to BCLAF1 in Human, Mouse, Rat
Applications
A04100 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
106122 MW
Background of BTF
Patatin-like phospholipase domain containing 6 (PNPLA6), also known as Neuropathy target esterase (NTE), is a human gene. Neuropathy target esterase (NTE) is involved in neural development and is the target for neurodegeneration induced by selected organophosphorus pesticides and chemical warfare agents. The genetic or chemical reduction of Nte activity results in a neurological phenotype of hyperactivity in mammals and indicate that EOPF toxicity occurs directly through inhibition of Nte without the requirement for Nte gain of function or aging.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using BTF Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using BTF Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For BCLAF1 (Source: Uniprot.org, NCBI)
Gene Name
BCLAF1
Full Name
Bcl-2-associated transcription factor 1
Weight
106122 MW
Superfamily
BCLAF1/THRAP3 family
Alternative Names
bcl-2-associated transcription factor 1; BCL2-associated transcription factor 1; Btf; BTFKIAA0164bK211L9.1 BCLAF1 BTF, bK211L9.1 BCL2 associated transcription factor 1 bcl-2-associated transcription factor 1|BCLAF1 and THRAP3 family member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCLAF1, check out the BCLAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCLAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BTF Antibody (A04100)
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No publications found for A04100
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-BTF Antibody
Question
We are interested in to test anti-BTF antibody A04100 on human testis uterus for research purposes, then I may be interested in using anti-BTF antibody A04100 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The products we sell, including anti-BTF antibody A04100, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-01
Question
Is this A04100 anti-BTF antibody reactive to the isotypes of BCLAF1?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The immunogen of A04100 anti-BTF antibody is Synthesized peptide derived from the Internal region of human BTF. at AA rangle: 250-330. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-06
Question
Is there a BSA free version of anti-BTF antibody A04100 available?
J. Mitchell
Verified customer
Asked: 2019-12-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BTF antibody A04100 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-02
Question
We are currently using anti-BTF antibody A04100 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-BTF antibody (A04100) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use your anti-BTF antibody for WB for human testis uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis uterus identification?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can see on the product datasheet, A04100 anti-BTF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
I have a question about product A04100, anti-BTF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04100 anti-BTF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
Please see the WB image, lot number and protocol we used for testis uterus using anti-BTF antibody A04100. Please let me know if you require anything else.
M. Kulkarni
Verified customer
Asked: 2019-08-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
Would anti-BTF antibody A04100 work for WB with testis uterus?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
According to the expression profile of testis uterus, BCLAF1 is highly expressed in testis uterus. So, it is likely that anti-BTF antibody A04100 will work for WB with testis uterus.
Boster Scientific Support
Answered: 2018-10-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis uterus using anti-BTF antibody A04100. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-30
Question
Does A04100 anti-BTF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Banerjee
Verified customer
Asked: 2018-06-05
Answer
As indicated on the product datasheet, A04100 anti-BTF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-05
Question
Is a blocking peptide available for product anti-BTF antibody (A04100)?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
We do provide the blocking peptide for product anti-BTF antibody (A04100). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-16
Question
I see that the anti-BTF antibody A04100 works with WB, what is the protocol used to produce the result images on the product page?
J. Johnson
Verified customer
Asked: 2014-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-12-25
Question
Does anti-BTF antibody A04100 work on zebrafish WB with prostate cancer?
O. Jones
Verified customer
Asked: 2014-04-08
Answer
Our lab technicians have not validated anti-BTF antibody A04100 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-BTF antibody A04100 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish prostate cancer in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-08
