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Product Info Summary
| SKU: | A02753-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BSCL2/Seipin Antibody Picoband®
View all Seipin/BSCL2 Antibodies
SKU/Catalog Number
A02753-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BSCL2/Seipin Antibody Picoband® catalog # A02753-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-BSCL2/Seipin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02753-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BSCL2/Seipin recombinant protein (Position: A87-P231).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02753-1 is reactive to BSCL2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
12252 MW
Background of Seipin/BSCL2
Seipin is a protein that in humans is encoded by the BSCL2 gene. This gene encodes the multi-pass transmembrane protein protein seipin. This protein localizes to the endoplasmic reticulum and may be important for lipid droplet morphology. Mutations in this gene have been associated with congenital generalized lipodystrophy type 2 or Berardinelli-Seip syndrome, a rare autosomal recessive disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HNRNPUL2 (heterogeneous nuclear ribonucleoprotein U-like 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02753-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, human U-87MG whole cell, human Hacat whole cell, rat testis tissue, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BSCL2/Seipin using anti-BSCL2/Seipin antibody (A02753-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSCL2/Seipin antigen affinity purified polyclonal antibody (Catalog # A02753-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSCL2/Seipin at approximately 46 kDa. The expected band size for BSCL2/Seipin is at 44 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BSCL2 (Source: Uniprot.org, NCBI)
Gene Name
BSCL2
Full Name
Seipin
Weight
12252 MW
Superfamily
seipin family
Alternative Names
Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; PPBP; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; NAP-2 Bscl2|2900097C17Rik, AI046355, Gng3, Gng3lg, s|Berardinelli-Seip congenital lipodystrophy 2 (seipin)|seipin|Berardinelli-Seip congenital lipodystrophy 2 homolog (seipin)|Bernardinelli-Seip congenital lipodystrophy 2 homolog|G protein gamma 3 linked|bernardinelli-Seip congenital lipodystrophy type 2 protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BSCL2, check out the BSCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BSCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BSCL2/Seipin Antibody Picoband® (A02753-1)
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