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Product Info Summary
| SKU: | A02412-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-BRD2 Antibody Picoband®
SKU/Catalog Number
A02412-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BRD2 Antibody Picoband® catalog # A02412-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02412-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BRD2 recombinant protein (Position: M1-Q214).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02412-1 is reactive to BRD2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
78948 MW
Background of BRD2
Bromodomain-containing protein 2 is a protein that in humans is encoded by the BRD2 gene. It is mapped to 6p21.32. This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02412-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human A431 whole cell, human Jurkat whole cell, human 293T whole cell
ICC/IF: A431 cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 3. Flow Cytometry analysis of 293T cells using anti-BRD2 antibody (A02412-1).
Overlay histogram showing 293T cells stained with A02412-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD2 Antibody (A02412-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of BRD2 using anti-BRD2 antibody (A02412-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD2 antigen affinity purified polyclonal antibody (Catalog # A02412-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD2 at approximately 110 kDa. The expected band size for BRD2 is at 88 kDa.
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Figure 2. IF analysis of BRD2 and Tubulin beta using anti-BRD2 antibody (A02412-1) and anti-Tubulin beta antibody (M05613-4).
BRD2 and Tubulin beta was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BRD2 Antibody (A02412-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For BRD2 (Source: Uniprot.org, NCBI)
Gene Name
BRD2
Full Name
Bromodomain-containing protein 2
Weight
78948 MW
Alternative Names
Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein; BRD2; KIAA9001; RING3 BRD2 BRD2-IT1, D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3 bromodomain containing 2 bromodomain-containing protein 2|BRD2 intronic transcript 1|female sterile homeotic-related gene 1|really interesting new gene 3 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRD2, check out the BRD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRD2 Antibody Picoband® (A02412-1)
Hello CJ!
A02412-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pharmacologic Targeting of BET Proteins Attenuates Hyperuricemic Nephropathy in Rats
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