Anti-BRD2 Antibody

Product Name

Anti-BRD2 Antibody

View all BRD2 Antibodies

SKU/Catalog Number

A02412

Size

100ul

Form

Liquid

Description

Boster Bio Anti-BRD2 Antibody catalog # A02412. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02412)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CD318 around the phosphorylation site of Y707.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02412 is reactive to BRD2 in Human

Applications

A02412 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

88061 MW

Background of BRD2

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)
Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)
Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of BRD2 using Anti-BRD2 Antibody (A02412).

ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-BRD2 Antibody A02412

Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2

Gene/Protein Information For BRD2 (Source: Uniprot.org, NCBI)

Alternative Names

bromodomain containing 2; bromodomain-containing 2; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSRG1FSH; KIAA9001FLJ31942; NAT; O27.1.1; Really interesting new gene 3 protein; RING3DKFZp686N0336; RNF3 BRD2 BRD2-IT1, D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3 bromodomain containing 2 bromodomain-containing protein 2|BRD2 intronic transcript 1|female sterile homeotic-related gene 1|really interesting new gene 3 protein

For more info on BRD2, check out the BRD2 Infographic

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In this infographic, you will see the following information for BRD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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