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Product Info Summary
| SKU: | A32264 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BRCC45 BABAM2 Antibody
SKU/Catalog Number
A32264
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BRCC45 BABAM2 Antibody (Catalog # A32264). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BRCC45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BRCC45 BABAM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32264)
Host
Rabbit
Contents
BRCC45 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BRCC45 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC45. The immunogen is located within the last 50 amino acids of BRCC45.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
It is predicted to not cross-react with other members of the cavin family.
Reactive Species
A32264 is reactive to BRE in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
43552 MW
Background of BRE
BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32264 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BRCC45 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with BRCC45 antibody at 1 μg/mL.
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Immunohistochemistry of BRCC45 in human brain tissue with BRCC45 antibody at 2.5 μg/mL.
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Immunofluorescence of BRCC45 in human brain tissue with BRCC45 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BRE (Source: Uniprot.org, NCBI)
Gene Name
BRE
Full Name
BRISC and BRCA1-A complex member 2
Weight
43552 MW
Superfamily
BABAM2 family
Alternative Names
BRCC4, BRCC45, BRCA1-A complex subunit BRE, Brain and reproductive organ-expressed protein BABAM2 BRCC4, BRCC45, BRE BRISC and BRCA1 A complex member 2 BRISC and BRCA1-A complex member 2|BRCA1-A complex subunit BRE|BRCA1/BRCA2-containing complex subunit 45|BRCA1/BRCA2-containing complex, subunit 4|brain and reproductive organ-expressed (TNFRSF1A modulator)|brain and reproductive organ-expressed protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRE, check out the BRE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRCC45 BABAM2 Antibody (A32264)
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