Anti-BRCC36 Antibody

Product Name

Anti-BRCC36 Antibody

View all BRCC3 Antibodies

SKU/Catalog Number

A04690

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BRCC36 Antibody (Catalog # A04690). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BRCC36 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04690)

Host

Rabbit

Contents

BRCC36 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.

Reactive Species

A04690 is reactive to BRCC3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

36072 MW

Background of BRCC3

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04690 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For BRCC3 (Source: Uniprot.org, NCBI)

Alternative Names

C6.1A, BRCC36, CXorf53, C6.1A, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36 BRCC3 BRCC36, C6.1A, CXorf53 BRCA1/BRCA2-containing complex subunit 3 lys-63-specific deubiquitinase BRCC36|BRCA1-A complex subunit BRCC36|BRCA1/BRCA2-containing complex subunit 36|BRISC complex subunit BRCC36

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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