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Product Info Summary
| SKU: | M06924-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BMP6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06924-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-BMP6 Rabbit Monoclonal Antibody catalog # M06924-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06924-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
21B45
Isotype
IgG
Immunogen
A synthesized peptide derived from human BMP6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06924-1 is reactive to BMP6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
31052 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06924-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human SiHa whole cell, human HEL whole cell, human 293T whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BMP6 using anti-BMP6 antibody (M06924-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP6 antigen affinity purified monoclonal antibody (Catalog # M06924-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP6 at approximately 42 kDa. The expected band size for BMP6 is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BMP6 (Source: Uniprot.org, NCBI)
Gene Name
BMP6
Full Name
Bone morphogenetic protein 6
Weight
31052 MW
Superfamily
TGF-beta family
Alternative Names
MAD2L1-binding protein;Caught by MAD2 protein;MAD2L1BP;CMT2, KIAA0110; BMP6 VGR, VGR1 bone morphogenetic protein 6 bone morphogenetic protein 6|VG-1-R|VG-1-related protein|vegetal related growth factor (TGFB-related)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMP6, check out the BMP6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMP6 Rabbit Monoclonal Antibody (M06924-1)
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