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Product Info Summary
| SKU: | M00321 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BMP4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00321
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-BMP4 Rabbit Monoclonal Antibody catalog # M00321. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00321)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BBB-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human BMP4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00321 is reactive to BMP4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
46555 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00321 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human COLO320 whole cell, human CACO-2 whole cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BMP4 using anti-BMP4 antibody (M00321).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified monoclonal antibody (Catalog # M00321) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP4 at approximately 47 kDa. The expected band size for BMP4 is at 47 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(M00321)
BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (M00321)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For BMP4 (Source: Uniprot.org, NCBI)
Gene Name
BMP4
Full Name
Bone morphogenetic protein 4
Weight
46555 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;BMP4;BMP2B, DVR4; BMP4 BMP2B, BMP2B1, MCOPS6, OFC11, ZYME bone morphogenetic protein 4 bone morphogenetic protein 4|bone morphogenetic protein 2B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMP4, check out the BMP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMP4 Rabbit Monoclonal Antibody (M00321)
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