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Product Info Summary
| SKU: | A10502 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BHLHA15 Antibody
SKU/Catalog Number
A10502
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BHLHA15 Antibody (Catalog # A10502). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-BHLHA15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10502)
Host
Rabbit
Contents
BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
BHLHA15 antibody is human, mouse and rat reactive.
Reactive Species
A10502 is reactive to BHLHA15 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20818 MW
Background of MIST1
BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10502 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide
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Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.
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Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BHLHA15 (Source: Uniprot.org, NCBI)
Gene Name
BHLHA15
Full Name
Class A basic helix-loop-helix protein 15
Weight
20818 MW
Alternative Names
MIST1, BHLHB8, MIST1, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, bHLHa15 Bhlha15|1810009C13Rik, Bhlhb, Bhlhb8, MIST-1, Mis, Mist1|basic helix-loop-helix family, member a15|class A basic helix-loop-helix protein 15|basic helix-loop-helix domain containing, class B, 8|muscle, intestine and stomach expression 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BHLHA15, check out the BHLHA15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BHLHA15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BHLHA15 Antibody (A10502)
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