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Product Info Summary
| SKU: | M04668 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-BHLH3 Antibody (N-term)
View all DEC2/SHARP1 Antibodies
SKU/Catalog Number
M04668
Size
50 µl
Description
Boster Bio Anti-BHLH3 Antibody (N-term) (Catalog # M04668). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BHLH3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04668)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2135CT82.1.84
Isotype
IgG2b,κ
Immunogen
This BHLH3 antibody is generated from a mouse immunized with a recombinant protein of human BHLH3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04668 is reactive to BHLHE41 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
50498 Da
Background of DEC2/SHARP1
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04668 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-BHLH3 Antibody (N-term) at 1:4000 dilution
Lane 1: HeLa whole cell lysate
Lane 2: RD whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate
Lane 7: Rat skeletal muscle tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: CHO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For BHLHE41 (Source: Uniprot.org, NCBI)
Gene Name
BHLHE41
Full Name
Class E basic helix-loop-helix protein 41
Weight
50498 Da
Alternative Names
Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, bHLHb3, Differentially expressed in chondrocytes protein 2, hDEC2, Enhancer-of-split and hairy-related protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2, SHARP1 Bhlhe41|6430520M22Rik, Bhlhb, Bhlhb2l, Bhlhb3, DEC2, Sh, Sharp1|basic helix-loop-helix family, member e41|class E basic helix-loop-helix protein 41|bHLH transcriptional factor Dec2|basic helix-loop-helix domain containing, class B3|class B basic helix-loop-helix protein 3|differentially expressed in chondrocytes protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BHLHE41, check out the BHLHE41 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BHLHE41: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BHLH3 Antibody (N-term) (M04668)
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