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Product Info Summary
| SKU: | A04668-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-BHLH3 Antibody (N-term)
View all DEC2/SHARP1 Antibodies
SKU/Catalog Number
A04668-2
Size
80 µl
Description
Boster Bio Anti-BHLH3 Antibody (N-term) (Catalog # A04668-2). Tested in WB, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BHLH3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04668-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04668-2 is reactive to BHLHE41 in Human, Mouse
Reconstitution
Calculated molecular weight
50498 Da
Background of DEC2/SHARP1
BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04668-2 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.
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Anti-BHLH3 Antibody (N-term) at 1:1000 dilution + human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-BHLH3 Antibody (N-term) at 1:2000 dilution + Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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BHLH3 Antibody (N-term) western blot analysis in RD cell line lysates (35ug/lane).This demonstrates the BHE41 antibody detected the BHE41 protein (arrow).
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BHLH3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For BHLHE41 (Source: Uniprot.org, NCBI)
Gene Name
BHLHE41
Full Name
Class E basic helix-loop-helix protein 41
Weight
50498 Da
Alternative Names
Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, bHLHb3, Differentially expressed in chondrocytes protein 2, hDEC2, Enhancer-of-split and hairy-related protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2, SHARP1 Bhlhe41|6430520M22Rik, Bhlhb, Bhlhb2l, Bhlhb3, DEC2, Sh, Sharp1|basic helix-loop-helix family, member e41|class E basic helix-loop-helix protein 41|bHLH transcriptional factor Dec2|basic helix-loop-helix domain containing, class B3|class B basic helix-loop-helix protein 3|differentially expressed in chondrocytes protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BHLHE41, check out the BHLHE41 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BHLHE41: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BHLH3 Antibody (N-term) (A04668-2)
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