Anti-Bfl-1 BCL2A1 Antibody

Product Name

Anti-Bfl-1 BCL2A1 Antibody

View all Bcl-2 related protein A1 Antibodies

SKU/Catalog Number

A03850

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bfl-1 BCL2A1 Antibody (Catalog # A03850). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Bfl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bfl-1 BCL2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03850)

Host

Rabbit

Contents

Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.

Reactive Species

A03850 is reactive to BCL2A1 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

20132 MW

Background of Bcl-2 related protein A1

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03850 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For BCL2A1 (Source: Uniprot.org, NCBI)

Alternative Names

GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5, GRS, Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5 BCL2A1 ACC-1, ACC-2, ACC1, ACC2, BCL2L5, BFL1, GRS, HBPA1 BCL2 related protein A1 bcl-2-related protein A1|bcl-2-like protein 5|bcl2-L-5|hematopoietic BCL2-related protein A1|hemopoietic-specific early response protein|protein BFL-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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