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Product Info Summary
| SKU: | M09008 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta II Tubulin Rabbit Monoclonal Antibody
View all Tubulin Beta 2C Antibodies
SKU/Catalog Number
M09008
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta II Tubulin Rabbit Monoclonal Antibody catalog # M09008. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta II Tubulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09008)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta II Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09008 is reactive to TUBB4B in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
50 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09008 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Beta II Tubulin using anti-Beta II Tubulin antibody (M09008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta II Tubulin antigen affinity purified monoclonal antibody (Catalog # M09008) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta II Tubulin at approximately 50 kDa. The expected band size for Beta II Tubulin is at 50 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse brain, using beta II Tubulin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TUBB4B (Source: Uniprot.org, NCBI)
Gene Name
TUBB4B
Full Name
Tubulin beta-4B chain
Weight
50 kDa
Superfamily
tubulin family
Alternative Names
Tubulin beta-4B chain;Tubulin beta-2 chain;Tubulin beta-2C chain;TUBB4B;TUBB2C; TUBB4B Beta2, LCAEOD, TUBB2, TUBB2C tubulin beta 4B class IVb tubulin beta-4B chain|class IVb beta tubulin|epididymis secretory sperm binding protein|tubulin beta-2 chain|tubulin beta-2C chain|tubulin, beta 2C|tubulin, beta, 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB4B, check out the TUBB4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta II Tubulin Rabbit Monoclonal Antibody (M09008)
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Customer Q&As
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7 Customer Q&As for Anti-beta II Tubulin Rabbit Monoclonal Antibody
Question
Would anti-beta II Tubulin Rabbit Monoclonal antibody M09008 work for WB with epithelium of bronchus?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
According to the expression profile of epithelium of bronchus, TUBB4B is highly expressed in epithelium of bronchus. So, it is likely that anti-beta II Tubulin Rabbit Monoclonal antibody M09008 will work for WB with epithelium of bronchus.
Boster Scientific Support
Answered: 2019-09-05
Question
Is there a BSA free version of anti-beta II Tubulin Rabbit Monoclonal antibody M09008 available?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-beta II Tubulin Rabbit Monoclonal antibody M09008 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-01
Question
Will M09008 anti-beta II Tubulin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
You can see on the product datasheet, M09008 anti-beta II Tubulin Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-29
Question
We are currently using anti-beta II Tubulin Rabbit Monoclonal antibody M09008 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The anti-beta II Tubulin Rabbit Monoclonal antibody (M09008) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-08
Question
I see that the anti-beta II Tubulin Rabbit Monoclonal antibody M09008 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-02
Question
Here is the WB image, lot number and protocol we used for epithelium of bronchus using anti-beta II Tubulin Rabbit Monoclonal antibody M09008. Please let me know if you require anything else.
G. Jha
Verified customer
Asked: 2013-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of bronchus using anti-beta II Tubulin Rabbit Monoclonal antibody M09008. Let me know if you need anything else.
S. Wu
Verified customer
Asked: 2013-01-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-07
