Anti-BDKRB2 Antibody Picoband™

Product Name

Anti-BDKRB2 Antibody Picoband™

View all Bradykinin RB2/BDKRB2 Antibodies

SKU/Catalog Number

PB10047

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BDKRB2 Antibody Picoband™ catalog # PB10047. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BDKRB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10047)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BDKRB2, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10047 is reactive to BDKRB2 in Human

Applications

PB10047 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

44461 MW

Background of Bradykinin RB2/BDKRB2

Bradykinin receptor B2 is a G-protein coupled receptor forbradykinin, encoded by the BDKRB2 gene in humans. This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB10047).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPG2 whole cell lysates，
Lane 3: MCF-7 whole cell lysates.
Lane 4: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (Catalog # PB10047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.

Gene/Protein Information For BDKRB2 (Source: Uniprot.org, NCBI)

Alternative Names

B2 bradykinin receptor; B2R; BDKRB2; BK-2 receptor; BK2; BK-2; BKR2; Bradykinin RB2; bradykinin receptor B2; BradykininRB2; BRB2; DKFZp686O088 BDKRB2 B2R, BK-2, BK2, BKR2, BRB2 bradykinin receptor B2 B2 bradykinin receptor|BK-2 receptor

For more info on BDKRB2, check out the BDKRB2 Infographic

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In this infographic, you will see the following information for BDKRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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