Anti-Bcl-G BCL2L14 Antibody

Product Name

Anti-Bcl-G BCL2L14 Antibody

View all Bcl G Antibodies

SKU/Catalog Number

A09347

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bcl-G BCL2L14 Antibody (Catalog # A09347). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Bcl-G antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bcl-G BCL2L14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09347)

Host

Rabbit

Contents

Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G. The immunogen is located within the first 50 amino acids of Bcl-G.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Although antibody should react with both isoforms, only the Bcl-GS protein has been observed

Reactive Species

A09347 is reactive to Bcl2l14 in Human, Mouse, Rat

Applications

A09347 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

36598 MW

Background of Bcl G

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 μg/mL.

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Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 μg/mL.

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Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G antibody at 10 μg/mL.

Gene/Protein Information For Bcl2l14 (Source: Uniprot.org, NCBI)

Alternative Names

apoptosis facilitator Bcl-2-like protein 14; Apoptosis regulator Bcl-G; Bcl2-L-14; BCL2-like 14 (apoptosis facilitator); BCL-G; BCLGbcl2-L-14 Bcl2l14|4930452K23Rik, 4933405K19Rik, 9030625M01Rik, AI429211, Bcl-G, Bclg|BCL2-like 14 (apoptosis facilitator)|apoptosis facilitator Bcl-2-like protein 14|apoptosis regulator BCL-G

For more info on Bcl2l14, check out the Bcl2l14 Infographic

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In this infographic, you will see the following information for Bcl2l14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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