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Product Info Summary
| SKU: | A07983 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BAMBI Antibody
SKU/Catalog Number
A07983
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAMBI Antibody (Catalog # A07983). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAMBI antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-BAMBI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07983)
Host
Rabbit
Contents
BAMBI antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI. The immunogen is located within amino acids 100 - 150 of BAMBI.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
BAMBI antibody is human, mouse and rat reactive.
Reactive Species
A07983 is reactive to BAMBI in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
29108 MW
Background of BAMBI/NMA
BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07983 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BAMBI in mouse lung tissue lysate with BAMBI antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of BAMBI in human lung tissue with BAMBI antibody at 5 μg/ml.
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Immunofluorescence of BAMBI in human lung tissue with BAMBI antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For BAMBI (Source: Uniprot.org, NCBI)
Gene Name
BAMBI
Full Name
BMP and activin membrane-bound inhibitor homolog
Weight
29108 MW
Superfamily
BAMBI family
Alternative Names
BMP and activin membrane-bound inhibitor homolog BAMBI NMA BMP and activin membrane bound inhibitor BMP and activin membrane-bound inhibitor homolog|non-metastatic gene A protein|putative transmembrane protein NMA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAMBI, check out the BAMBI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAMBI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAMBI Antibody (A07983)
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