Anti-BAFF TNFSF13B Antibody

Product Name

Anti-BAFF TNFSF13B Antibody

View all BAFF/BLyS/TNFSF13B Antibodies

SKU/Catalog Number

A01257-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BAFF TNFSF13B Antibody (Catalog # A01257-1). Tested in ELISA, WB, ICC, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BAFF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BAFF TNFSF13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01257-1)

Host

Rabbit

Contents

BAFF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF. The immunogen is located within the last 50 amino acids of BAFF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.

Reactive Species

A01257-1 is reactive to TNFSF13B in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

31223 MW

Background of BAFF/BLyS/TNFSF13B

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01257-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.25-1 μg/mL; ICC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Immunohistochemistry Validation of BAFF in Murine Cardiac Transplants at Rejection (Ye et al., 2004)
BAFF expression detected by anti-BAFF antibodies (A01257-1) was upregulated in intragraft leukocytes due to rejection at 7 days after heart transplant.