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Product Info Summary
| SKU: | M01257 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Human |
| Host: | Rat |
| Application: | IHC |
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Product info
Product Name
Anti-BAFF Monoclonal Antibody (Buffy-2)
View all BAFF/BLyS/TNFSF13B Antibodies
SKU/Catalog Number
M01257
Size
100µg
Form
Liquid. In PBS containing 0.02% sodium azide.
Description
Boster Bio Anti-BAFF Monoclonal Antibody (Buffy-2) catalog # M01257. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
+4°C
Cite This Product
Anti-BAFF Monoclonal Antibody (Buffy-2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01257)
Host
Rat
Contents
Liquid. In PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Buffy-2
Immunogen
Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01257 is reactive to TNFSF13B in Human
Reconstitution
Calculated molecular weight
31.223kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01257 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure: Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2.Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
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Schematic structure of rhsBAFF (aa. 83-285). MAb to BAFF (Buffy-2) recognizes the TNF-homology domain.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF13B (Source: Uniprot.org, NCBI)
Gene Name
TNFSF13B
Full Name
Tumor necrosis factor ligand superfamily member 13B
Weight
31.223kDa
Superfamily
tumor necrosis factor family
Alternative Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor n TNFSF13B BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4 TNF superfamily member 13b tumor necrosis factor ligand superfamily member 13B|ApoL related ligand TALL-1|B-cell-activating factor|B-lymphocyte stimulator|Delta4 BAFF|TNF and ApoL-related leukocyte expressed ligand 1|TNF homolog that activates apoptosis|delta BAFF|dendritic cell-derived TNF-like molecule|epididymis secretory sperm binding protein|tumor necrosis factor (ligand) superfamily, member 13b|tumor necrosis factor (ligand) superfamily, member 20|tumor necrosis factor ligand 7A|tumor necrosis factor superfamily member 13b|tumor necrosis factor-like protein ZTNF4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF13B, check out the TNFSF13B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF13B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAFF Monoclonal Antibody (Buffy-2) (M01257)
Hello CJ!
M01257 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of B-cell activating factor in acute lymphoblastic leukemia patients
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-BAFF Monoclonal Antibody (Buffy-2)
Question
Is there a BSA free version of anti-BAFF Monoclonal antibody (Buffy-2) M01257 available?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BAFF Monoclonal antibody (Buffy-2) M01257 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-06
Question
Can you help my question with product M01257, anti-BAFF Monoclonal antibody (Buffy-2) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01257 anti-BAFF Monoclonal antibody (Buffy-2) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-BAFF Monoclonal antibody (Buffy-2) for IHC for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
As indicated on the product datasheet, M01257 anti-BAFF Monoclonal antibody (Buffy-2) has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-11
Question
Is a blocking peptide available for product anti-BAFF Monoclonal antibody (Buffy-2) (M01257)?
R. Collins
Verified customer
Asked: 2017-06-27
Answer
We do provide the blocking peptide for product anti-BAFF Monoclonal antibody (Buffy-2) (M01257). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-27
Question
See attached the WB image, lot number and protocol we used for blood using anti-BAFF Monoclonal antibody (Buffy-2) M01257. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-26
Question
Is this M01257 anti-BAFF Monoclonal antibody (Buffy-2) reactive to the isotypes of TNFSF13B?
K. Carter
Verified customer
Asked: 2014-08-04
Answer
The immunogen of M01257 anti-BAFF Monoclonal antibody (Buffy-2) is Recombinant human soluble BAFF (aa 83-285) (Prod. No. ALX-522-025) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-04
