Product Info Summary
SKU: | A30528 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-BAF250B ARID1B Antibody
SKU/Catalog Number
A30528
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BAF250B ARID1B Antibody (Catalog# A30528). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAF250B ARID1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30528)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BAF250B. Immunogen sequence location: 1371-1420
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30528 is reactive to ARID1B in Human, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
236123 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30528 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from LOVO cells, using BAF250B Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HUVEC cells, using BAF250B Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ARID1B (Source: Uniprot.org, NCBI)
Gene Name
ARID1B
Full Name
AT-rich interactive domain-containing protein 1B
Weight
236123 MW
Alternative Names
ARID1B;BAF250B;DAN15;KIAA1235;OSA2;AT-rich interactive domain-containing protein 1B;ARID domain-containing protein 1B;BRG1-associated factor 250b;BAF250B;BRG1-binding protein hELD/OSA1;Osa homolog 2;hOsa2;p250R ARID1B 6A3-5, BAF250B, BRIGHT, CSS1, DAN15, ELD/OSA1, MRD12, OSA2, P250R AT-rich interaction domain 1B AT-rich interactive domain-containing protein 1B|ARID domain-containing protein 1B|AT rich interactive domain 1B (SWI1-like)|BRG1-associated factor 250b|BRG1-binding protein ELD/OSA1|ELD (eyelid)/OSA protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARID1B, check out the ARID1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARID1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAF250B ARID1B Antibody (A30528)
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No publications found for A30528
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Customer Q&As
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3 Customer Q&As for Anti-BAF250B ARID1B Antibody
Question
I was wanting to use your anti-BAF250B antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can see on the product datasheet, A30528 anti-BAF250B antibody has been tested for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-BAF250B antibody A30528 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on pig tissues as well?
E. Thomas
Verified customer
Asked: 2015-11-20
Answer
The anti-BAF250B antibody (A30528) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-20
Question
Can you help my question with product A30528, anti-BAF250B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Moore
Verified customer
Asked: 2014-09-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30528 anti-BAF250B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-30