Anti-BACH1 BRIP1 Antibody

Product Name

Anti-BACH1 BRIP1 Antibody

View all BRIP1/FANCJ Antibodies

SKU/Catalog Number

A01995

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-BACH1 BRIP1 Antibody (Catalog # A01995). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-BACH1 BRIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01995)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01995 is reactive to BRIP1 in Human

Applications

A01995 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

98809 MW

Background of BRIP1/FANCJ

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:44,000
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of BRIP1 using anti-BRIP1 antibody (A01995).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRIP1 antigen affinity purified polyclonal antibody (Catalog # A01995) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BRIP1.

Gene/Protein Information For BRIP1 (Source: Uniprot.org, NCBI)

Alternative Names

ATP-dependent RNA helicase BRIP1; BACH1; BACH1BRCA1/BRCA2-associated helicase 1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRIP1; EC 3.6.1; EC 3.6.4.13; FACJ; FANCJ; FANCJFanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ BRIP1 BACH1, FANCJ, OF BRCA1 interacting helicase 1 Fanconi anemia group J protein|ATP-dependent RNA helicase BRIP1|BRCA1 interacting protein C-terminal helicase 1|BRCA1-associated C-terminal helicase 1|BRCA1-binding helicase-like protein BACH1|BRCA1/BRCA2-associated helicase 1|FANCJ helicase

For more info on BRIP1, check out the BRIP1 Infographic

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In this infographic, you will see the following information for BRIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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