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Product Info Summary
| SKU: | A02347-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-BACH1.3/BACH1 Antibody Picoband®
SKU/Catalog Number
A02347-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BACH1.3/BACH1 Antibody Picoband® catalog # A02347-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-BACH1.3/BACH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02347-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BACH1.3/BACH1 recombinant protein (Position: N102-K657).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02347-2 is reactive to BACH1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
81.958kDa
Background of BACH1
Transcription regulator protein BACH1 is a protein that in humans is encoded by the BACH1 gene. This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02347-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEK293 whole cell, human Raji whole cell, human HepG2 whole cell, human Hela whole cell, human MCF-7 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BACH1.3/BACH1 using anti-BACH1.3/BACH1 antibody (A02347-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACH1.3/BACH1 antigen affinity purified polyclonal antibody (Catalog # A02347-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACH1.3/BACH1 at approximately 100 kDa. The expected band size for BACH1.3/BACH1 is at 100 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-BACH1.3/BACH1 antibody (A02347-2).
Overlay histogram showing HepG2 cells stained with A02347-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BACH1.3/BACH1 Antibody (A02347-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For BACH1 (Source: Uniprot.org, NCBI)
Gene Name
BACH1
Full Name
Transcription regulator protein BACH1
Weight
81.958kDa
Superfamily
bZIP family
Alternative Names
Acid-sensing ion channel 1; ASIC1;Amiloride-sensitive cation channel 2, neuronal; Brain sodium channel 2; BNaC2; ASIC1; ACCN2, BNAC2 BACH1 BACH-1, BTBD24 BTB domain and CNC homolog 1 transcription regulator protein BACH1|BTB and CNC homology 1, basic leucine zipper transcription factor 1|basic region leucine zipper transcriptional regulator BACH1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BACH1, check out the BACH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BACH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BACH1.3/BACH1 Antibody Picoband® (A02347-2)
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