Product Info Summary
SKU: | M00196-3 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: OTI1E9]
SKU/Catalog Number
M00196-3
Size
100 µl
Description
Boster Bio CD80 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9). Catalog# M00196-3. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196-3)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1E9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00196-3 is reactive to CD80 in Dog, Human, Monkey, Rat
Reconstitution
Calculated molecular weight
33.048kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00196-3 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either CD80 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD80 antibody (M00196-3)
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Anti-CD80 mouse monoclonal antibody (M00196-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)
Gene Name
CD80
Full Name
T-lymphocyte activation antigen CD80
Weight
33.048kDa
Alternative Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD80, check out the CD80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: OTI1E9] (M00196-3)
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