Product Info Summary
SKU: | M00075-5 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | IP, IF, WB |
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Product info
Product Name
Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI3A7]
SKU/Catalog Number
M00075-5
Size
100 µl
Description
Boster Bio Anti-BRAF mouse monoclonal antibody, clone OTI3A7 (formerly 3A7). Catalog# M00075-5. Tested in IF, IP, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI3A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00075-5)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3A7
Isotype
IgG2a
Immunogen
Full-length protein expressed in 293T cell transfected with human BRAF expression vector
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00075-5 is reactive to BRAF in Dog, Human, Monkey, Mouse, Rat
Applications
M00075-5 is guaranteed for IP, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
![m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 wb testing 1 m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m00075-5-anti-b-raf-braf-mouse-monoclonal-antibody-clone-id-oti3a7-wb-testing-1.jpg)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
![m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 wb testing 2 m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 wb testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00075-5-anti-b-raf-braf-mouse-monoclonal-antibody-clone-id-oti3a7-wb-testing-2.jpg)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
![m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 if testing 3 m00075 5 anti b raf braf mouse monoclonal antibody clone id oti3a7 if testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00075-5-anti-b-raf-braf-mouse-monoclonal-antibody-clone-id-oti3a7-if-testing-3.jpg)
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Anti-BRAF mouse monoclonal antibody (M00075-5) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
Superfamily
protein kinase superfamily
Alternative Names
94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
![BRAF infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI3A7] (M00075-5)
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