Product Info Summary
SKU: | A00986 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Axin-1 AXIN1 Antibody
SKU/Catalog Number
A00986
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Axin-1 AXIN1 Antibody (Catalog # A00986). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AXIN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Axin-1 AXIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00986)
Host
Rabbit
Contents
AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Reactive Species
A00986 is reactive to AXIN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
95635 MW
Background of Axin-1
AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00986 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.
Click image to see more details
Immunofluorescence of AXIN1 in human brain tissue with AXIN1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AXIN1 (Source: Uniprot.org, NCBI)
Gene Name
AXIN1
Full Name
Axin-1
Weight
95635 MW
Alternative Names
AXIN, PPP1R49, AXIN, Axin-1, Axis inhibition protein 1, hAxin AXIN1 AXIN, PPP1R49 axin 1 axin-1|axis inhibition protein 1|axis inhibitor 1|fused, mouse, homolog of|protein phosphatase 1, regulatory subunit 49
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AXIN1, check out the AXIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AXIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Axin-1 AXIN1 Antibody (A00986)
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