Anti-Autophagy-related protein 101 ATG101 Antibody

Product Name

Anti-Autophagy-related protein 101 ATG101 Antibody

View all ATG101 Antibodies

SKU/Catalog Number

A07713

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Autophagy-related protein 101 ATG101 Antibody (Catalog # A07713). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG101 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Autophagy-related protein 101 ATG101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07713)

Host

Rabbit

Contents

ATG101 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG101 antibody was raised against a 16 amino acid synthetic peptide near the center of human ATG101. The immunogen is located within amino acids 80 - 130 of ATG101.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Reactive Species

A07713 is reactive to C12ORF44 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

25003 MW

Background of ATG101

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07713 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For C12ORF44 (Source: Uniprot.org, NCBI)

Alternative Names

C12orf44, C12orf44, PP894, Autophagy-related protein 101 ATG101 C12orf44 autophagy related 101 autophagy-related protein 101|Atg13-interacting protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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