Product Info Summary
SKU: | PB9483 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ATX2/ATXN2 Antibody Picoband®
SKU/Catalog Number
PB9483
PB0505 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATX2/ATXN2 Antibody Picoband® catalog # PB9483. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATX2/ATXN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9483)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ATX2, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9483 is reactive to ATXN2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
140 kDa
Calculated molecular weight
140283 MW
Background of Ataxin-2
Ataxin-2, the protein encoded by the ATXN2 gene, contains a polyglutamine tract, long expansions (greater than 33 repeats) of which result in spinocerebellar ataxia-2 (SCA2), an autosomal dominant form of olivopontocerebellar atrophy. The gene for spinocerebellar ataxia type 2 (SCA2) has been mapped to 12q24.1. Ataxin-2 associates with L- and T-plastin and that overexpression of ataxin-2 leads to accumulation of T-plastin in mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9483 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human Hela whole cell, human K562 whole cell, human A549 whole cell, rat brain tissue, mouse brain tissue, mouse liver tissue
IHC: human breast cancer tissue, human colonic adenoma cancer tissue, human glioma tissue, human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATX2/ATXN2 antigen affinity purified polyclonal antibody (Catalog # PB9483) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATX2/ATXN2 at approximately 140 kDa. The expected band size for ATX2/ATXN2 is at 140 kDa.
Click image to see more details
Figure 2. IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483).
ATX2/ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483).
ATX2/ATXN2 was detected in a paraffin-embedded section of human colonic adenoma cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483).
ATX2/ATXN2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483).
ATX2/ATXN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ATXN2 (Source: Uniprot.org, NCBI)
Gene Name
ATXN2
Full Name
Ataxin-2
Weight
140283 MW
Superfamily
ataxin-2 family
Alternative Names
Ataxin-2;Spinocerebellar ataxia type 2 protein;Trinucleotide repeat-containing gene 13 protein;ATXN2;ATX2, SCA2, TNRC13; ATXN2 ATX2, SCA2, TNRC13 ataxin 2 ataxin-2|spinocerebellar ataxia type 2 protein|trinucleotide repeat-containing gene 13 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATXN2, check out the ATXN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATXN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATX2/ATXN2 Antibody Picoband® (PB9483)
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Customer Q&As
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9 Customer Q&As for Anti-ATX2/ATXN2 Antibody Picoband®
Question
Will PB9483 work in IHC-P?
Verified customer
Asked: 2022-04-14
Answer
Yes, Anti-ATX2/ATXN2 Antibody Picoband™ (PB9483) is guaranteed for IHC-P.
Boster Scientific Support
Answered: 2022-04-14
Question
Will PB9483 work in WB?
Verified customer
Asked: 2022-04-14
Answer
Yes, Anti-ATX2/ATXN2 Antibody Picoband™ (PB9483) is guaranteed for WB.
Boster Scientific Support
Answered: 2022-04-14
Question
I see that the anti-ATX2/ATXN2 antibody PB9483 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ATX2/ATXN2 antibody PB9483. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Do you have a BSA free version of anti-ATX2/ATXN2 antibody PB9483 available?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATX2/ATXN2 antibody PB9483 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-22
Question
I was wanting to use your anti-ATX2/ATXN2 antibody for ICC for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
It shows on the product datasheet, PB9483 anti-ATX2/ATXN2 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-13
Question
We are currently using anti-ATX2/ATXN2 antibody PB9483 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
N. Edwards
Verified customer
Asked: 2014-11-18
Answer
The anti-ATX2/ATXN2 antibody (PB9483) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-18
Question
I have a question about product PB9483, anti-ATX2/ATXN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Krishna
Verified customer
Asked: 2013-12-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9483 anti-ATX2/ATXN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-03
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-ATX2/ATXN2 antibody PB9483. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2013-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-28