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Product Info Summary
| SKU: | M03598 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody
SKU/Catalog Number
M03598
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody catalog # M03598. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03598)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAFC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATP5A1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03598 is reactive to ATP5A1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
59751 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03598 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of ATP5A1 expression in HepG2 cell lysate;.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ATP5A1 (Source: Uniprot.org, NCBI)
Gene Name
ATP5A1
Full Name
ATP synthase subunit alpha, mitochondrial
Weight
59751 MW
Superfamily
ATPase alpha/beta chains family
Alternative Names
ATP synthase subunit alpha, mitochondrial;ATP5A1;ATP5A, ATP5AL2, ATPM; ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1 ATP synthase F1 subunit alpha ATP synthase subunit alpha, mitochondrial|ATP synthase alpha chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle|ATP sythase (F1-ATPase) alpha subunit|epididymis secretory sperm binding protein Li 123m|mitochondrial ATP synthetase, oligomycin-resistant
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5A1, check out the ATP5A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody (M03598)
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No publications found for M03598
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody
Question
We are currently using anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
H. Baker
Verified customer
Asked: 2019-05-27
Answer
The anti-ATP5A1/Atp5A Rabbit Monoclonal antibody (M03598) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-27
Question
I see that the anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-08
Question
Is this M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody reactive to the isotypes of Atp5a1?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
The immunogen of M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody is A synthesized peptide derived from human ATP5A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brown adipose tissue using anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-15
Question
Can you help my question with product M03598, anti-ATP5A1/Atp5A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Johnson
Verified customer
Asked: 2013-12-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-10
