Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

ATP5F1A antibody

Boster Bio Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody catalog # M03598. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M03598
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

View all ATP5F1A Antibodies

SKU/Catalog Number

M03598

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody catalog # M03598. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03598)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAFC-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ATP5A1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03598 is reactive to ATP5A1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

45 kDa

Calculated molecular weight

59751 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M03598 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ATP5A1 (Source: Uniprot.org, NCBI)

Gene Name

ATP5A1

Full Name

ATP synthase subunit alpha, mitochondrial

Weight

59751 MW

Superfamily

ATPase alpha/beta chains family

Alternative Names

ATP synthase subunit alpha, mitochondrial;ATP5A1;ATP5A, ATP5AL2, ATPM; ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1 ATP synthase F1 subunit alpha ATP synthase subunit alpha, mitochondrial|ATP synthase alpha chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle|ATP sythase (F1-ATPase) alpha subunit|epididymis secretory sperm binding protein Li 123m|mitochondrial ATP synthetase, oligomycin-resistant

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATP5A1, check out the ATP5A1 Infographic

ATP5A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATP5A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ATP5A1/Atp5A Rabbit Monoclonal Antibody

Question

We are currently using anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

H. Baker

Verified customer

Asked: 2019-05-27

Answer

The anti-ATP5A1/Atp5A Rabbit Monoclonal antibody (M03598) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-27

Question

I see that the anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-08

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-08

Question

Is this M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody reactive to the isotypes of Atp5a1?

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

The immunogen of M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody is A synthesized peptide derived from human ATP5A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-01

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brown adipose tissue using anti-ATP5A1/Atp5A Rabbit Monoclonal antibody M03598. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-15

Question

Can you help my question with product M03598, anti-ATP5A1/Atp5A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Johnson

Verified customer

Asked: 2013-12-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03598 anti-ATP5A1/Atp5A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-12-10

Order DetailsPrice
M03598

100μL

$370
M03598-30ul

30μL sample

$99
M03598-carrier-free-1ml

Carrier Free

$2590

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M03598
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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