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Product Info Summary
| SKU: | A10328 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ATP11B Antibody
SKU/Catalog Number
A10328
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATP11B Antibody (Catalog # A10328). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ATP11B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATP11B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10328)
Host
Rabbit
Contents
ATP11B Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
ATP11B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11B. The immunogen is located within amino acids 290 - 340 of ATP11B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of UPK1B are known to exist; this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members.
Reactive Species
A10328 is reactive to ATP11B in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
134190 MW
Background of ATP11B
ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10328 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATP11B antibody can be used for detection of ATP11B by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATP11B in K562 cell tissue lysate with ATP11B antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ATP11B in K562 cells with ATP11B antibody at 10 μg/mL.
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Immunofluorescence of ATP11B in K562 cells with ATP11B antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATP11B (Source: Uniprot.org, NCBI)
Gene Name
ATP11B
Full Name
Probable phospholipid-transporting ATPase IF
Weight
134190 MW
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATPase class VI type 11B; ATPase IR; ATPase, class VI, type 11B; ATPIFKIAA0956probable phospholipid-transporting ATPase IF; ATPIRMGC46576; DKFZp434J238; DKFZp434N1615; EC 3.6.3; EC 3.6.3.1 ATP11B ATPIF, ATPIR ATPase phospholipid transporting 11B (putative) probable phospholipid-transporting ATPase IF|ATPase IR|ATPase, class VI, type 11B|P4-ATPase flippase complex alpha subunit ATP11B|truncated ATPase 11B protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP11B, check out the ATP11B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP11B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP11B Antibody (A10328)
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