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Product Info Summary
| SKU: | M01800 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATGL / PNPLA2 Rabbit Monoclonal Antibody
View all PEDFR/PNPLA2/ATGL Antibodies
SKU/Catalog Number
M01800
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ATGL / PNPLA2 Rabbit Monoclonal Antibody catalog # M01800. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATGL / PNPLA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01800)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23P38
Isotype
IgG
Immunogen
A synthesized peptide derived from human ATGL / PNPLA2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01800 is reactive to PNPLA2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
50976 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01800 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human A431 whole cell, human 293T whole cell, human SiHa whole cell, human HepG2 whole cell, rat C6 whole cell, rat heart tissue, mouse NIH/3T3 whole cell, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PNPLA2 (Source: Uniprot.org, NCBI)
Gene Name
PNPLA2
Full Name
Patatin-like phospholipase domain-containing protein 2
Weight
50976 MW
Alternative Names
Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein;TUNP;HNRNPK;HNRPK; PNPLA2 1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2, TTS2, iPLA2zeta patatin like phospholipase domain containing 2 patatin-like phospholipase domain-containing protein 2|IPLA2-zeta|TTS2.2|adipose triglyceride lipase|calcium-independent phospholipase A2|desnutrin|mutant patatin-like phospholipase domain containing 2|pigment epithelium-derived factor|transport-secretion protein 2.2|triglyceride hydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PNPLA2, check out the PNPLA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PNPLA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATGL / PNPLA2 Rabbit Monoclonal Antibody (M01800)
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