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Product Info Summary
| SKU: | M00371 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATF4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00371
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ATF4 Rabbit Monoclonal Antibody catalog # M00371. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00371)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HCI-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ATF4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00371 is reactive to ATF4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
39,55 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00371 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50
Positive Control
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (M00371).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified monoclonal antibody (Catalog # M00371) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 55 kDa. The expected band size for ATF4 is at 39,55 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using ATF4 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of 293 cells, using ATF4 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATF4 (Source: Uniprot.org, NCBI)
Gene Name
ATF4
Full Name
Cyclic AMP-dependent transcription factor ATF-4
Weight
39,55 kDa
Superfamily
bZIP family
Alternative Names
Cyclic AMP-dependent transcription factor ATF-4;cAMP-dependent transcription factor ATF-4;Activating transcription factor 4;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2;DNA-binding protein TAXREB67;Tax-responsive enhancer element-binding protein 67;TaxREB67;ATF4;CREB2, TXREB; ATF4 CREB-2, CREB2, TAXREB67, TXREB activating transcription factor 4 cyclic AMP-dependent transcription factor ATF-4|DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF4, check out the ATF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF4 Rabbit Monoclonal Antibody (M00371)
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Customer Q&As
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17 Customer Q&As for Anti-ATF4 Rabbit Monoclonal Antibody
Question
Will anti-ATF4 Rabbit Monoclonal antibody M00371 work on goat IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
Our lab technicians have not tested anti-ATF4 Rabbit Monoclonal antibody M00371 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ATF4 Rabbit Monoclonal antibody M00371 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-21
Question
My colleagues were satisfied with the WB result of your anti-ATF4 Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is ATF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
From what I have seen in literature, leukemic t-cell does express ATF4. Generally ATF4 expresses in cytoplasm. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2020-02-28
Question
Please see the WB image, lot number and protocol we used for fibroblast using anti-ATF4 Rabbit Monoclonal antibody M00371. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
My question regards to test anti-ATF4 Rabbit Monoclonal antibody M00371 on human fibroblast for research purposes, then I may be interested in using anti-ATF4 Rabbit Monoclonal antibody M00371 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Carter
Verified customer
Asked: 2019-12-09
Answer
The products we sell, including anti-ATF4 Rabbit Monoclonal antibody M00371, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-09
Question
We are currently using anti-ATF4 Rabbit Monoclonal antibody M00371 for rat tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-ATF4 Rabbit Monoclonal antibody (M00371) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
I have a question about product M00371, anti-ATF4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00371 anti-ATF4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-12
Question
I was wanting to use your anti-ATF4 Rabbit Monoclonal antibody for WB for human fibroblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fibroblast identification?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
You can see on the product datasheet, M00371 anti-ATF4 Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-ATF4 Rabbit Monoclonal antibody M00371. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-27
Question
Is a blocking peptide available for product anti-ATF4 Rabbit Monoclonal antibody (M00371)?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We do provide the blocking peptide for product anti-ATF4 Rabbit Monoclonal antibody (M00371). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-17
Question
Will M00371 anti-ATF4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
You can see on the product datasheet, M00371 anti-ATF4 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-06
Question
Does anti-ATF4 Rabbit Monoclonal antibody M00371 work for WB with fibroblast?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
According to the expression profile of fibroblast, ATF4 is highly expressed in fibroblast. So, it is likely that anti-ATF4 Rabbit Monoclonal antibody M00371 will work for WB with fibroblast.
Boster Scientific Support
Answered: 2018-09-05
Question
We have observed staining in mouse placenta urinary bladder. Do you have any suggestions? Is anti-ATF4 Rabbit Monoclonal antibody supposed to stain placenta urinary bladder positively?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
Based on literature placenta urinary bladder does express ATF4. Based on Uniprot.org, ATF4 is expressed in lower esophagus, fibroblast, leukemic t-cell, colon, lung, placenta urinary bladder, cervix carcinoma, among other tissues. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2017-10-30
Question
I would like using your anti-ATF4 Rabbit Monoclonal antibody for positive regulation of apoptotic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
Thanks for your inquiry. This M00371 anti-ATF4 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-17
Question
We ordered your anti-ATF4 Rabbit Monoclonal antibody for ICC on leukemic t-cell in the past. I am using rat, and We want to use the antibody for WB next. I would like examining leukemic t-cell as well as colon in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
B. Thomas
Verified customer
Asked: 2016-11-24
Answer
I took a look at the website and datasheets of our anti-ATF4 Rabbit Monoclonal antibody and it seems that M00371 has been tested on rat in both ICC and WB. Thus M00371 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-11-24
Question
I see that the anti-ATF4 Rabbit Monoclonal antibody M00371 works with WB, what is the protocol used to produce the result images on the product page?
E. Kulkarni
Verified customer
Asked: 2015-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-05
Question
Is there a BSA free version of anti-ATF4 Rabbit Monoclonal antibody M00371 available?
W. Miller
Verified customer
Asked: 2014-05-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATF4 Rabbit Monoclonal antibody M00371 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-05-21
Question
Is this M00371 anti-ATF4 Rabbit Monoclonal antibody reactive to the isotypes of ATF4?
S. Dhar
Verified customer
Asked: 2013-03-15
Answer
The immunogen of M00371 anti-ATF4 Rabbit Monoclonal antibody is A synthesized peptide derived from human ATF4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-15
