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Product Info Summary
| SKU: | A10294-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ARSE/ASE Antibody Picoband®
SKU/Catalog Number
A10294-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARSE/ASE Antibody Picoband® catalog # A10294-3. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARSE/ASE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10294-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat ARSE/ASE, which shares 77.8% amino acid (aa) sequence identity with human ARSE/ASE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10294-3 is reactive to Arse in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
41409 MW
Background of Arse
Arylsulfatase E, also known as ARSE, is an enzyme that, in humans, is encoded by the ARSE gene. Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the Y chromosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10294-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Positive Control
WB: rat Rh35 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARSE/ASE using anti-ARSE/ASE antibody (A10294-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat Rh35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE antigen affinity purified polyclonal antibody (Catalog # A10294-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARSE/ASE at approximately 70KD. The expected band size for ARSE/ASE is at 66KD
Protein Target Info & Infographic
Gene/Protein Information For Arse (Source: Uniprot.org, NCBI)
Gene Name
Arse
Full Name
Weight
41409 MW
Alternative Names
Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Arse, check out the Arse Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Arse: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARSE/ASE Antibody Picoband® (A10294-3)
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