Product Info Summary
SKU: | A06839 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-ArfGAP3 Antibody
SKU/Catalog Number
A06839
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ArfGAP3 Antibody (Catalog # A06839). Tested in IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ArfGAP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06839)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06839 is reactive to ARFGAP3 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
37807 MW
Background of ARFGAP3
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus; however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06839 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000
IF Microscopy: 1:100
IP: User optimized
WB: 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody.Tissue:HeLa Whole Cell.Fixation:MeOH. Antigen retrieval:not required.Primary antibody:ArfGAP3 antibodyat 1:100 for 1 h at RT.Secondary antibody:Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization:ArfGAP3 is cytoplasmic.Staining:ArfGAP3 as green fluorescent sinal.
Click image to see more details
Figure 2. Western blot analysis of ARFGAP3 using anti-ARFGAP3 antibody (A06839).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARFGAP3 antigen affinity purified polyclonal antibody (Catalog # A06839) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ARFGAP3.
Protein Target Info & Infographic
Gene/Protein Information For ARFGAP3 (Source: Uniprot.org, NCBI)
Gene Name
ARFGAP3
Full Name
ADP-ribosylation factor GTPase-activating protein 3
Weight
37807 MW
Alternative Names
ArfGAP3, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP3, Arf-GAP, ArfGAP ARFGAP3 ARFGAP1 ADP ribosylation factor GTPase activating protein 3 ADP-ribosylation factor GTPase-activating protein 3|ADP-ribosylation factor GTPase activating protein 1|ARF GAP 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARFGAP3, check out the ARFGAP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARFGAP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ArfGAP3 Antibody (A06839)
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3 Customer Q&As for Anti-ArfGAP3 Antibody
Question
Does A06839 anti-ArfGAP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
You can see on the product datasheet, A06839 anti-ArfGAP3 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-01
Question
I was wanting to use to test anti-ArfGAP3 antibody A06839 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ArfGAP3 antibody A06839 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The products we sell, including anti-ArfGAP3 antibody A06839, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-ArfGAP3 antibody A06839 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
O. Mangal
Verified customer
Asked: 2017-06-09
Answer
The anti-ArfGAP3 antibody (A06839) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-09