Product Info Summary
SKU: | A04959 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ARFGAP1 Antibody Picoband®
SKU/Catalog Number
A04959
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARFGAP1 Antibody Picoband® catalog # A04959. Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARFGAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04959)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04959 is reactive to ARFGAP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
44668 MW
Background of ARFGAP1
ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. And this gene is mapped to 20q13.33. The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04959 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human MOLT-4 whole cell, human Caco-2 whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat brain tissue
ICC/IF: CACO-2 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ARFGAP1 using anti-ARFGAP1 antibody (A04959).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARFGAP1 antigen affinity purified polyclonal antibody (Catalog # A04959) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARFGAP1 at approximately 45 kDa. The expected band size for ARFGAP1 is at 45 kDa.
Click image to see more details
Figure 2. IHC analysis of ARFGAP1 using anti-ARFGAP1 antibody (A04959).
ARFGAP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARFGAP1 Antibody (A04959) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ARFGAP1 using anti-ARFGAP1 antibody (A04959).
ARFGAP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARFGAP1 Antibody (A04959) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of ARFGAP1 using anti-ARFGAP1 antibody (A04959).
ARFGAP1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARFGAP1 Antibody (A04959) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-ARFGAP1 antibody (A04959).
Overlay histogram showing HepG2 cells stained with A04959 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARFGAP1 Antibody (A04959, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ARFGAP1 (Source: Uniprot.org, NCBI)
Gene Name
ARFGAP1
Full Name
ADP-ribosylation factor GTPase-activating protein 1
Weight
44668 MW
Alternative Names
ADP-ribosylation factor GTPase-activating protein 1;ARF GAP 1;ADP-ribosylation factor 1 GTPase-activating protein;ARF1 GAP;ARF1-directed GTPase-activating protein;ARFGAP1;ARF1GAP; ARFGAP1 ARF1GAP, HRIHFB2281 ADP ribosylation factor GTPase activating protein 1 ADP-ribosylation factor GTPase-activating protein 1|ARF1-directed GTPase-activating protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARFGAP1, check out the ARFGAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARFGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARFGAP1 Antibody Picoband® (A04959)
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Customer Q&As
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13 Customer Q&As for Anti-ARFGAP1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-ARFGAP1 antibody (A04959)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
We do provide the blocking peptide for product anti-ARFGAP1 antibody (A04959). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ARFGAP1 antibody A04959. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
Would anti-ARFGAP1 antibody A04959 work on monkey WB with thymus?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
Our lab technicians have not validated anti-ARFGAP1 antibody A04959 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-ARFGAP1 antibody A04959 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
I would like to test anti-ARFGAP1 antibody A04959 on rat liver for research purposes, then I may be interested in using anti-ARFGAP1 antibody A04959 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The products we sell, including anti-ARFGAP1 antibody A04959, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-23
Question
My question regarding product A04959, anti-ARFGAP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04959 anti-ARFGAP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-30
Question
Here is the WB image, lot number and protocol we used for liver using anti-ARFGAP1 antibody A04959. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-ARFGAP1 antibody A04959 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The anti-ARFGAP1 antibody (A04959) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-23
Question
I see that the anti-ARFGAP1 antibody A04959 works with WB, what is the protocol used to produce the result images on the product page?
R. Moore
Verified customer
Asked: 2019-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-17
Question
Does A04959 anti-ARFGAP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
As indicated on the product datasheet, A04959 anti-ARFGAP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-16
Question
I was wanting to use your anti-ARFGAP1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
G. Williams
Verified customer
Asked: 2018-12-10
Answer
You can see on the product datasheet, A04959 anti-ARFGAP1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-10
Question
Do you have a BSA free version of anti-ARFGAP1 antibody A04959 available?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ARFGAP1 antibody A04959 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-08
Question
Is this A04959 anti-ARFGAP1 antibody reactive to the isotypes of ARFGAP1?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
The immunogen of A04959 anti-ARFGAP1 antibody is E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-07
Question
Will anti-ARFGAP1 antibody A04959 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
According to the expression profile of liver, ARFGAP1 is highly expressed in liver. So, it is likely that anti-ARFGAP1 antibody A04959 will work for WB with liver.
Boster Scientific Support
Answered: 2018-06-06