Product Info Summary
SKU: | M02759 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8)
SKU/Catalog Number
M02759
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) catalog # M02759. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02759)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
10G8
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human ARA9 recombinant protein (Position: D91-H330). Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02759 is reactive to AIP in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
37.636kDa
Background of AIP/ARA9
AIP, also known as, ARA9 or XAP-2, is a protein that in humans is encoded by the AIP gene. This gene is mapped to 11q13.2. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. AIP may play a positive role in aryl hydrocarbon receptor-mediated signalling possibly by influencing its receptivity for ligand and/or its nuclear targeting. It has been shown that AIP is the cellular negative regulator of the hepatitis B virus (HBV) X protein. AIP mutations may be the cause of a familial form of acromegaly, familial isolated pituitary adenoma (FIPA).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02759 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human HELA whole cell, human HEPG2 whole cell, human placenta tissue, human CACO-2 whole cell, human U20S whole cell, monkey COS-7 whole cell, human K562 whole cell, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat spleen tissue, mouse brain tissue, mouse HEPA1-6 whole cell
IHC: human intestinal cancer tissue, human tonsil tissue, rat spleen tissue
ICC/IF: A431 cell
FCM: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human U20S whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates,
Lane 7: human K562 whole cell lysates,
Lane 8: rat heart tissue lysates,
Lane 9: rat skeletal muscle tissue lysates,
Lane 10: rat brain tissue lysates,
Lane 11: rat spleen tissue lysates,
Lane 12: mouse brain tissue lysates,
Lane 13: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARA9/AIP antigen affinity purified monoclonal antibody (Catalog # M02759) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ARA9/AIP at approximately 38KD. The expected band size for ARA9/AIP is at 38KD.
Click image to see more details
Figure 2. IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759).
ARA9/AIP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759).
ARA9/AIP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759).
ARA9/AIP was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759).
ARA9/AIP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of A549 cells using anti-ARA9/AIP antibody (M02759).
Overlay histogram showing A549 cells stained with M02759 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ARA9/AIP Antibody (M02759, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For AIP (Source: Uniprot.org, NCBI)
Gene Name
AIP
Full Name
AH receptor-interacting protein
Weight
37.636kDa
Alternative Names
H receptor-interacting protein; AIP; Aryl-hydrocarbon receptor-interacting protein; HBV X-associated protein 2; XAP-2; Immunophilin homolog ARA9; AIP; XAP2 AIP ARA9, FKBP16, FKBP37, PITA1, SMTPHN, XAP-2, XAP2 aryl hydrocarbon receptor interacting protein AH receptor-interacting protein|Ah receptor activated 9|FK506-binding protein 37|FKBP prolyl isomerase 16|HBV X-associated protein 2|X-associated protein-2|aryl hydrocarbon receptor-associated protein 9|hepatitis B virus X-associated cellular protein 2|immunophilin homolog ARA9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AIP, check out the AIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) (M02759)
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Customer Q&As
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5 Customer Q&As for Anti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8)
Question
I have a question about product M02759, anti-ARA9/AIP antibody (monoclonal, 10G8). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02759 anti-ARA9/AIP antibody (monoclonal, 10G8), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ARA9/AIP antibody (monoclonal, 10G8) M02759. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-05
Question
Does M02759 anti-ARA9/AIP antibody (monoclonal, 10G8) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It shows on the product datasheet, M02759 anti-ARA9/AIP antibody (monoclonal, 10G8) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
Is this M02759 anti-ARA9/AIP antibody (monoclonal, 10G8) reactive to the isotypes of AIP?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The immunogen of M02759 anti-ARA9/AIP antibody (monoclonal, 10G8) is E.coli-derived human ARA9 recombinant protein (Position: D91-H330). Human ARA9 shares 95% amino acid (aa) sequence identity with both mouse and rat ARA9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-13
Question
Would anti-ARA9/AIP antibody (monoclonal, 10G8) M02759 work on zebrafish WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
Our lab technicians have not validated anti-ARA9/AIP antibody (monoclonal, 10G8) M02759 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ARA9/AIP antibody (monoclonal, 10G8) M02759 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-06