Anti-Aquaporin 10/AQP10 Antibody Picoband®

Product Name

Anti-Aquaporin 10/AQP10 Antibody Picoband®

View all Aquaporin-10 Antibodies

SKU/Catalog Number

PA2095

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aquaporin 10/AQP10 Antibody catalog # PA2095. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aquaporin 10/AQP10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2095)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2095 is reactive to AQP10 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

31 kDa

Calculated molecular weight

31763 MW

Background of Aquaporin-10

AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3 (53%), 9 (52%), and 7 (43%). These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP (AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2095 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: COLO320 Cell

Validation Images & Assay Conditions

Gene/Protein Information For AQP10 (Source: Uniprot.org, NCBI)

Alternative Names

Aquaporin-10;AQP-10;Aquaglyceroporin-10;Small intestine aquaporin;AQP10; AQP10 AQPA_HUMAN aquaporin 10 aquaporin-10|AQP-10|aquaglyceroporin-10|small intestine aquaporin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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