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Product Info Summary
| SKU: | PB9811 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aquaporin 0/MIP Antibody Picoband®
View all Aquaporin-0 Antibodies
SKU/Catalog Number
PB9811
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 0/MIP Antibody Picoband® catalog # PB9811. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 0/MIP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9811)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 0, different from the related mouse and rat sequences by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9811 is reactive to MIP in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
28122 MW
Background of Aquaporin-0
Lens fiber major intrinsic protein, also called MIP26 or MP26, is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9811 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat eye ball tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Aquaporin 0 using anti-Aquaporin 0 antibody (PB9811).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: rat eye ball tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 0 antigen affinity purified polyclonal antibody (Catalog # PB9811) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 0 at approximately 28 kDa. The expected band size for Aquaporin 0 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MIP (Source: Uniprot.org, NCBI)
Gene Name
MIP
Full Name
Lens fiber major intrinsic protein
Weight
28122 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
Lens fiber major intrinsic protein;Aquaporin-0;MIP26;MP26;MIP;AQP0; MIP AQP0, CTRCT15, LIM126, MP26, MIP major intrinsic protein of lens fiber lens fiber major intrinsic protein|aquaporin 0
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIP, check out the MIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 0/MIP Antibody Picoband® (PB9811)
Hello CJ!
PB9811 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis
Mei X, Wang HX, Li JS, Liu XH, Lu XF, Li Y, Zhang WY, Tian YG. BMC Complement Altern Med. 2017 Apr 17;17(1):216. doi: 10.1186/s12906-017-1736-x. Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling.
Bruns I, Cadeddu Rp, Brueckmann I, Fr??bel J, Geyh S, B??st S, Fischer Jc, Roels F, Wilk Cm, Schildberg Fa, H??nerlit??rkoglu An, Zilkens C, J??ger M, Steidl U, Zohren F, Fenk R, Kobbe G, Brors B, Czibere A, Schroeder T, Trumpp A, Haas R. Blood. 2...
Sun Y, Zhao Y, Yao J, Zhao L, Wu Z, Wang Y, Pan D, Miao H, Guo Q, Lu N. Biochem Pharmacol. 2015 Mar 15;94(2):142-54. Doi: 10.1016/J.Bcp.2015.02.002. Epub 2015 Feb 10. Wogonoside Protects Against Dextran Sulfate Sodium-Induced Experimental Colitis ...
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
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Customer Q&As
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1 Customer Q&As for Anti-Aquaporin 0/MIP Antibody Picoband®
Question
We are currently using anti-Aquaporin 0/MIP antibody PB9811 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The anti-Aquaporin 0/MIP antibody (PB9811) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
