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Product Info Summary
| SKU: | PA2110 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Aquaporin 0/MIP Antibody
View all Aquaporin-0 Antibodies
SKU/Catalog Number
PA2110
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aquaporin 0/MIP Antibody catalog # PA2110. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 0/MIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2110)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0, identical to the related rat sequence, and different from the related human sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2110 is reactive to MIP in Human, Mouse, Rat
Applications
PA2110 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
27891 MW
Background of Aquaporin-0
Lens fiber major intrinsic protein also called MIP26 or MP26 is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-MIP antibody, PA2110, Western blotting
All lanes: Anti MIP (PA2110) at 0.5ug/ml
Lane 1: Mouse Spleen Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted bind size: 28KD
Observed bind size: 50KD
Protein Target Info & Infographic
Gene/Protein Information For MIP (Source: Uniprot.org, NCBI)
Gene Name
MIP
Full Name
Lens fiber major intrinsic protein
Weight
27891 MW
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
aquaporin 0; lens fiber major intrinsic protein; major intrinsic protein of lens fiber; MP26 MIP AQP0, CTRCT15, LIM126, MP26, MIP major intrinsic protein of lens fiber lens fiber major intrinsic protein|aquaporin 0
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIP, check out the MIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 0/MIP Antibody (PA2110)
Hello CJ!
PA2110 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis
Mei X, Wang HX, Li JS, Liu XH, Lu XF, Li Y, Zhang WY, Tian YG. BMC Complement Altern Med. 2017 Apr 17;17(1):216. doi: 10.1186/s12906-017-1736-x. Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling.
Bruns I, Cadeddu Rp, Brueckmann I, Fr??bel J, Geyh S, B??st S, Fischer Jc, Roels F, Wilk Cm, Schildberg Fa, H??nerlit??rkoglu An, Zilkens C, J??ger M, Steidl U, Zohren F, Fenk R, Kobbe G, Brors B, Czibere A, Schroeder T, Trumpp A, Haas R. Blood. 2...
Sun Y, Zhao Y, Yao J, Zhao L, Wu Z, Wang Y, Pan D, Miao H, Guo Q, Lu N. Biochem Pharmacol. 2015 Mar 15;94(2):142-54. Doi: 10.1016/J.Bcp.2015.02.002. Epub 2015 Feb 10. Wogonoside Protects Against Dextran Sulfate Sodium-Induced Experimental Colitis ...
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Aquaporin 0/MIP Antibody
Question
I was wanting to use your anti-Aquaporin 0/MIP antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
As indicated on the product datasheet, PA2110 anti-Aquaporin 0/MIP antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Is this PA2110 anti-Aquaporin 0/MIP antibody reactive to the isotypes of MIP?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The immunogen of PA2110 anti-Aquaporin 0/MIP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0(246-263aa NGQPEGTGEPVELKTQAL), identical to the related rat sequence, and different from the related human sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-11
Question
I see that the anti-Aquaporin 0/MIP antibody PA2110 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-07
Question
Will PA2110 anti-Aquaporin 0/MIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Li
Verified customer
Asked: 2018-10-31
Answer
It shows on the product datasheet, PA2110 anti-Aquaporin 0/MIP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-31
Question
Is a blocking peptide available for product anti-Aquaporin 0/MIP antibody (PA2110)?
S. Banerjee
Verified customer
Asked: 2017-02-27
Answer
We do provide the blocking peptide for product anti-Aquaporin 0/MIP antibody (PA2110). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-27
Question
My question regarding product PA2110, anti-Aquaporin 0/MIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Patel
Verified customer
Asked: 2015-08-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2110 anti-Aquaporin 0/MIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-18
