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Product Info Summary
| SKU: | A07960-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-APP-1/PABPC4 Antibody Picoband®
SKU/Catalog Number
A07960-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-APP-1/PABPC4 Antibody Picoband® catalog # A07960-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-APP-1/PABPC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07960-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human APP-1/PABPC4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07960-2 is reactive to PABPC4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
49229 MW
Background of PABPC4
Polyadenylate-binding protein 4 (PABPC4) is a protein that in humans is encoded by the PABPC4 gene. Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein has also been found to interact with coronavirus nucleocapsid proteins and is thought to inhibit coronavirus replication.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07960-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse
Positive Control
WB: human Hela whole cell, human 293T whole cell, human PC-3 whole cell, human THP-1 whole cell, human RT4 whole cell, human A431 whole cell, human Daudi whole cell, rat testis tissue, rat heart tissue, rat brain tissue, rat L6 whole cell, mouse heart tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human cervical cancer tissue, human tonsil tissue
ICC/IF: SiHa cell
IF: human chronic tonsillitis tissue, human ovary cancer tissue
FCM: HEL cell, RAW2647 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human RT4 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human Daudi whole cell lysates,
Lane 8: rat testis tissue lysates,
Lane 9: rat heart tissue lysates,
Lane 10: rat brain tissue lysates,
Lane 11: rat L6 whole cell lysates,
Lane 12: mouse heart tissue lysates,
Lane 13: mouse brain tissue lysates,
Lane 14: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP-1/PABPC4 antigen affinity purified polyclonal antibody (Catalog # A07960-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP-1/PABPC4 at approximately 75 kDa. The expected band size for APP-1/PABPC4 is at 71 kDa.
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Figure 2. IHC analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
APP-1/PABPC4 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
APP-1/PABPC4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
APP-1/PABPC4 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 5. Flow Cytometry analysis of HEL cells using anti-APP-1/PABPC4 antibody (A07960-2).
Overlay histogram showing HEL cells stained with A07960-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (A07960-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 6. Flow Cytometry analysis of RAW264.7 cells using anti-APP-1/PABPC4 antibody (A07960-2).
Overlay histogram showing RAW264.7 cells stained with A07960-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (A07960-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 7. IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
APP-1/PABPC4 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 8. IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2).
APP-1/PABPC4 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PABPC4 (Source: Uniprot.org, NCBI)
Gene Name
PABPC4
Full Name
Polyadenylate-binding protein 4
Weight
49229 MW
Superfamily
polyadenylate-binding protein type-1 family
Alternative Names
DAZ-associated protein 1; Deleted in azoospermia-associated protein 1; DAZAP1 PABPC4 APP-1, APP1, PABP4, iPABP poly(A) binding protein cytoplasmic 4 polyadenylate-binding protein 4|PABP-4|activated-platelet protein 1|inducible poly(A)-binding protein|poly(A) binding protein, cytoplasmic 4 (inducible form)|poly(A)-binding protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PABPC4, check out the PABPC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PABPC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APP-1/PABPC4 Antibody Picoband® (A07960-2)
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