Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

Product Name

Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

View all ARC/NOL3 Antibodies

SKU/Catalog Number

PA1793

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1793)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1793 is reactive to NOL3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

80 kDa

Calculated molecular weight

22629 MW

Background of ARC/NOL3

NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1793 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: SMMC Cell, A549 Cell, U87 Cell, HELA Cell, MCF-7 Cell, Rat Liver Tissue,

Validation Images & Assay Conditions

Gene/Protein Information For NOL3 (Source: Uniprot.org, NCBI)

Alternative Names

Nucleolar protein 3 ;Apoptosis repressor with CARD ;Muscle-enriched cytoplasmic protein ;Myp ;Nucleolar protein of 30 kDa ;Nop30 ;NOL3 ;ARC , NOP ; NOL3 ARC, FCM, MYOCL1, MYP, NOP, NOP30 nucleolar protein 3 nucleolar protein 3|muscle-enriched cytoplasmic protein|nucleolar protein 3 (apoptosis repressor with CARD domain)|nucleolar protein of 30 kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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