Product Info Summary
SKU: | A00346-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-APG7 ATG7 Antibody
SKU/Catalog Number
A00346-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APG7 ATG7 Antibody (Catalog # A00346-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APG7 ATG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00346-1)
Host
Rabbit
Contents
APG7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.
Reactive Species
A00346-1 is reactive to ATG7 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
77960 MW
Background of ATG7
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00346-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5
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Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 μg/mL.
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Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)
Gene Name
ATG7
Full Name
Ubiquitin-like modifier-activating enzyme ATG7
Weight
77960 MW
Superfamily
ATG7 family
Alternative Names
GSA7, APG7L, APG7-LIKE, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, APG7-like ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG7, check out the ATG7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APG7 ATG7 Antibody (A00346-1)
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