Product Info Summary
SKU: | A07065 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-APC10 ANAPC10 Antibody
SKU/Catalog Number
A07065
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APC10 ANAPC10 Antibody (Catalog # A07065). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APC10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APC10 ANAPC10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07065)
Host
Rabbit
Contents
APC10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APC10 antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10. The immunogen is located within amino acids 60 - 110 of APC10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5.
Reactive Species
A07065 is reactive to ANAPC10 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
21252 MW
Background of Apc10
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07065 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APC10 antibody can be used for detection of APC10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APC10 in mouse heart tissue with APC10 antibody at 5 μg/mL.
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Immunofluorescence of APC10 in mouse heart tissue with APC10 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ANAPC10 (Source: Uniprot.org, NCBI)
Gene Name
ANAPC10
Full Name
Anaphase-promoting complex subunit 10
Weight
21252 MW
Superfamily
APC10 family
Alternative Names
DOC1, APC10, Anaphase-promoting complex subunit 10, Cyclosome subunit 10 ANAPC10 APC10, DOC1 anaphase promoting complex subunit 10 anaphase-promoting complex subunit 10|cyclosome subunit 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANAPC10, check out the ANAPC10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANAPC10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC10 ANAPC10 Antibody (A07065)
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