Anti-AP-2 TFAP2A Antibody

Product Name

Anti-AP-2 TFAP2A Antibody

View all AP2 alpha Antibodies

SKU/Catalog Number

A01167-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-AP-2 TFAP2A Antibody (Catalog # A01167-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AP-2 TFAP2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01167-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human AP-2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01167-1 is reactive to TFAP2A in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

48062 MW

Background of AP2 alpha

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

ShunJiang Yu, J. Biol. Chem., May 2003; 278: 19597 - 19602.
TT Chen, Mol. Cell. Biol., Jun 1997; 17: 3056 - 3064.
Oscar B. Goodman, J. Biol. Chem., Oct 1995; 270: 23768.
Didier Jean, J. Biol. Chem., Jun 1998; 273: 16501

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01167-1 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For TFAP2A (Source: Uniprot.org, NCBI)

Alternative Names

Transcription factor AP-2-alpha;AP2-alpha;AP-2 transcription factor;Activating enhancer-binding protein 2-alpha;Activator protein 2;AP-2;TFAP2A;AP2TF, TFAP2; TFAP2A AP-2, AP-2alpha, AP2TF, BOFS, TFAP2 transcription factor AP-2 alpha transcription factor AP-2-alpha|AP-2 transcription factor|activating enhancer-binding protein 2-alpha|activator protein 2|transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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