Product Info Summary
SKU: | A12867 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-ANKRD30A/Ny Br 1 Antibody
SKU/Catalog Number
A12867
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ANKRD30A/Ny Br 1 Antibody catalog # A12867. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANKRD30A/Ny Br 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12867)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human AN30A. AA range:1041-1090
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12867 is reactive to ANKRD30A in Human
Reconstitution
Calculated molecular weight
158835 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12867 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using ANKRD30A Polyclonal antibody.
Click image to see more details
Western blot analysis of lysates from 293 and COLO cells, using AN30A Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ANKRD30A (Source: Uniprot.org, NCBI)
Gene Name
ANKRD30A
Full Name
Ankyrin repeat domain-containing protein 30A
Weight
158835 MW
Alternative Names
Ankyrin repeat domain-containing protein 30A;Serologically defined breast cancer antigen NY-BR-1;ANKRD30A; ANKRD30A NY-BR-1 ankyrin repeat domain 30A ankyrin repeat domain-containing protein 30A|serologically defined breast cancer NY-BR-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANKRD30A, check out the ANKRD30A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANKRD30A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANKRD30A/Ny Br 1 Antibody (A12867)
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No publications found for A12867
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Customer Q&As
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4 Customer Q&As for Anti-ANKRD30A/Ny Br 1 Antibody
Question
Would anti-ANKRD30A/Ny Br 1 antibody A12867 work on pig WB with epithelium of mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Our lab technicians have not tested anti-ANKRD30A/Ny Br 1 antibody A12867 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ANKRD30A/Ny Br 1 antibody A12867 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig epithelium of mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of mammary gland using anti-ANKRD30A/Ny Br 1 antibody A12867. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-11
Question
I have a question about product A12867, anti-ANKRD30A/Ny Br 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Moore
Verified customer
Asked: 2017-07-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12867 anti-ANKRD30A/Ny Br 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-17
Question
Is this A12867 anti-ANKRD30A/Ny Br 1 antibody reactive to the isotypes of ANKRD30A?
T. Kulkarni
Verified customer
Asked: 2013-10-25
Answer
The immunogen of A12867 anti-ANKRD30A/Ny Br 1 antibody is Synthesized peptide derived from the C-terminal region of human ANKRD30A. at AA rangle: 1010-1090. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-25