Click image to see more details
Product Info Summary
| SKU: | A00146-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Angiogenin/Ang Antibody Picoband®
View all Angiogenin Antibodies
SKU/Catalog Number
A00146-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiogenin/Ang Antibody Picoband® catalog # A00146-3. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Angiogenin/Ang Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00146-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Angiogenin/Ang recombinant protein (Position: Q25-L145).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00146-3 is reactive to Ang in Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
16550 MW
Background of Angiogenin
Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Predicted to enable several functions, including actin binding activity; heparin binding activity; and protein homodimerization activity. Involved in liver development. Predicted to be located in several cellular components, including growth cone; neuronal cell body; and nucleolus. Predicted to be part of angiogenin-PRI complex. Human ortholog(s) of this gene implicated in diabetic retinopathy; myocardial infarction; neurodegenerative disease (multiple); and prostate cancer. Orthologous to human ANG (angiogenin).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00146-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Positive Control
WB: rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Angiogenin/Ang using anti-Angiogenin/Ang antibody (A00146-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiogenin/Ang antigen affinity purified polyclonal antibody (Catalog # A00146-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiogenin/Ang at approximately 25 kDa. The expected band size for Angiogenin/Ang is at 17-22 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Ang (Source: Uniprot.org, NCBI)
Gene Name
Ang
Full Name
Angiogenin
Weight
16550 MW
Superfamily
pancreatic ribonuclease family
Alternative Names
Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5; ANG ALS9, HEL168, RAA1, RNASE4, RNASE5 angiogenin angiogenin|RNase 5|angiogenin, ribonuclease, RNase A family, 5|epididymis luminal protein 168|ribonuclease 5|ribonuclease A A1|ribonuclease A family member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ang, check out the Ang Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ang: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiogenin/Ang Antibody Picoband® (A00146-3)
Hello CJ!
No publications found for A00146-3
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Angiogenin/Ang Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Angiogenin/Ang Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
