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Product Info Summary
| SKU: | A00994-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody
View all AMPK alpha 1 Antibodies
SKU/Catalog Number
A00994-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody catalog # A00994-2. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2B7
Isotype
IgG1
Immunogen
Purified recombinant fragment of human AMPKα1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00994-2 is reactive to PRKAA1 in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
64009 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00994-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400
Validation Images & Assay Conditions
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Western Blot (WB) analysis using AMPKalpha1 Monoclonal antibody against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
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Immunohistochemistry (IHC) analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) with DAB staining using AMPKalpha1 Monoclonal antibody.
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Flow cytometric (FCM) analysis of PC-2 cells using AMPKalpha1 Monoclonal antibody (green) and negative control (purple).
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Immunofluorescence (IF) analysis of NTERA-2 cells using AMPKalpha1 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA analysis of AMPKalpha1 antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRKAA1 (Source: Uniprot.org, NCBI)
Gene Name
PRKAA1
Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
Weight
64009 MW
Superfamily
protein kinase superfamily
Alternative Names
5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;2.7.11.1 ;Acetyl-CoA carboxylase kinase;ACACA kinase;2.7.11.27 ;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;2.7.11.31 ;Tau-protein kinase PRKAA1;2.7.11.26;PRKAA1;AMPK1; PRKAA1 AMPK, AMPKa1 protein kinase AMP-activated catalytic subunit alpha 1 5-AMP-activated protein kinase catalytic subunit alpha-1|5-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|protein kinase, AMP-activated, alpha 1 catalytic subunit|tau-protein kinase PRKAA1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAA1, check out the PRKAA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody (A00994-2)
Hello CJ!
A00994-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hou S, Zheng F, Li Y, Gao L, Zhang J. Int J Mol Sci. 2014 Aug 26;15(9):15026-43. Doi: 10.3390/Ijms150915026. The Protective Effect Of Glycyrrhizic Acid On Renal Tubular Epithelial Cell Injury Induced By High Glucose.
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Customer Q&As
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5 Customer Q&As for Anti-AMPK alpha1 PRKAA1 Monoclonal Antibody
Question
Do you have a BSA free version of anti-AMPK alpha1 Monoclonal antibody A00994-2 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AMPK alpha1 Monoclonal antibody A00994-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
Does anti-AMPK alpha1 Monoclonal antibody A00994-2 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of liver, PRKAA1 is highly expressed in liver. So, it is likely that anti-AMPK alpha1 Monoclonal antibody A00994-2 will work for WB with liver.
Boster Scientific Support
Answered: 2020-01-06
Question
I have a question about product A00994-2, anti-AMPK alpha1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00994-2 anti-AMPK alpha1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-31
Question
Will A00994-2 anti-AMPK alpha1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Anderson
Verified customer
Asked: 2017-03-06
Answer
You can see on the product datasheet, A00994-2 anti-AMPK alpha1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-06
Question
We are interested in to test anti-AMPK alpha1 Monoclonal antibody A00994-2 on rat liver for research purposes, then I may be interested in using anti-AMPK alpha1 Monoclonal antibody A00994-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Verified customer
Asked: 2013-10-02
Answer
The products we sell, including anti-AMPK alpha1 Monoclonal antibody A00994-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-02
